Diet induced obesity in rats pdf free

In this context, a useful tool in preclinical research to study the interaction between genes and diet is represented by rats genetically prone to diet induced obesity dio and their diet resistant dr counterpart levin et al. Controlled highfat diet induces an obese syndrome in rats. Control males are housed in the same location, also at reduced density, and are fed nih31m diet control males can also be generated using a low fat purified diet upon request. The diet induced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets.

To achieve diet induced obesity in normal rats, harlan spraguedawley rats, purchased from charles river laboratories raleigh, nc were employed. Diet induced obesity in rats is also reported to impact the brain in rats 24, as well as alter serum adipokine levels 25,26. After weaning, rats were fed either a normal or highfat diet for 12 weeks. Expression of new loci associated with obesity in diet induced obese rats. Mar 18, 2019 diet induced obese dio rats have altered stress hpa axis activity compared to diet resistant dr rats when chronically exposed to a highfat hf diet. Greater dietinduced obesity in rats consuming sugar solution. Dawley strain female rats 6 weeks old were divided into four groups. During 3 mo on a highenergy cm diet, twothirds of the male spraguedawley rats a. Raybould3 3department of anatomy, physiology, and cell biology, university of california davis school of veterinary medicine, davis.

The global prevalence of overweight and obesity has reached epidemic proportions. At the age of 3 weeks, male long evans rats were assigned to the high. The highfat diet resulted in 20% increased body weight. Thus, rats consuming the hf diet gained increased body fat relative to rats eating either of two quite different lf diets. Highfat diet induced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. Postnatal diet induced obesity in rats upregulates systemic and adipose tissue glucocorticoid metabolism during development and in adulthood sandrine boulluciocca, anne dutour, viviane guillaume, vincent achard, charles oliver, michel grino. The increase in free fatty acids flux to the liver from an expanded. Rats genetically prone to diet induced obesity dio and their counterpart diet resistant dr were fed a chow diet, and their response to damphetamine on intracranial selfstimulation and food intake were assessed. Dietinduced obesity in rats leads to a decrease in sperm motility article pdf available in reproductive biology and endocrinology 91.

In addition, hfhf and hfd induced obesity associated with hyperleptinaeemia and endothelial dysfunction. Many rat strains show variability in weight gain when fed a highenergy diet 21. Cromolyn sodium described as a mast cell stabilizer and chlorpheniramine maleate used as an antihistaminic and prevents allergic. Highfat dietinduced obesity in animal models niloofar hariri and louise thibault school of dietetics and human nutrition, mcgill university, quebec, canada epidemiological studies have shown a positive relationship between dietary fat intake and obesity. The hs diet induced increased visceral adipose tissue without increased body weight, reduced glucose disposal rates, and increased hepatic glucose output during the hyperinsulinemiceuglycemic clamp, increased plasma glucose during the. Modeling dietinduced obesity with obesityprone rats. Pdf energy expenditure in rats with dietinduced obesity. Jul 10, 2012 society for the study of ingestive behavior. Rodent model for the study of obesity related disorders 387rev assoc med bras 2012. In the past decades, obesity and associated metabolic complications have reached epidemic proportions. It was an animal experimental study and was carried out in the of pharmacology and. Prenatal stress or highfat diet increases susceptibility. Activation of the systemic and adipose reninangiotensin system in rats with dietinduced obesity and hypertension. Diet induced obesity in spraguedawley rats causes microvascular and neural dysfunction.

Fdio rats represent a novel and potentially useful model for the study of diet induced obesity and insulin resistance. In rodents, basal dopamine levels in the nac shell are lower in rats that are susceptible to diet. Keywords spraguedawley rat, dietinduced obesity, toxicology parameters, bone toxicity. Highfat diet hfd induced obesity in rats is considered to be a reliable tool for the evaluation of antiobesity activity. Effects of food pattern change and physical exercise on cafeteria dietinduced obesity in female rats volume 108 issue 8 jeferson f. The first description of a highfat diet to induce obesity by a nutritional intervention.

This study aimed to investigate the anti obesity effects of ginseng and the alternation of gut microbiota composition in highfat diet hfd induced obesity. The impact of the mitochondriatargeted antioxidant mitoq was evaluated in the cardiac alterations associated with obesity. Effects of food pattern change and physical exercise on. The effect of dietinduced obesity on toxicological parameters in. Initiation and perpetuation of obesity and obesity.

Pdf dietinduced obesity in rats leads to a decrease in. The study comprises 5 groups with 10 animals in each group. It is intended to mimic the most common cause of obesity in humans. Thus, inflammation, as well as other comorbidities, may provide a. Antiobesity activity of liraglutide on high fat diet. Rats were fed a standard or high fat diet for up to 32 weeks. Butyrate and propionate protect against dietinduced obesity. Highfat diet induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation skip to main content thank you for visiting. Since rats and mice show a similar relationship, they are considered an appropriate model for studying dietary obesity. Shortchain fatty acids protect against highfat dietinduced. A unique dio rat model to evaluate your drugs on obesity and cardiometabolic complications key benefits demonstrate the efficacy of your drugs in the physiogenex free choice diet induced obese dio rat.

Group represented the normal control in which the animals were feed on a normal diet npd and had free access to water. Results are consistent with other reports of diet induced obesity but only after 8 weeks of a hypercholesterolemic diet. Young male spraguedawley rats were fed chow or a modified highsucrose hs diet for 20 weeks. Effects of different diets used in dietinduced obesity models on. The total calories contained in 100 g of each diet were 349 and 365 kcal in the cg and hs, respectively. Beneficial effects of cocoa, coffee, green tea, and. Additionally, plasma ot levels were reported to be decreased in diet induced obese mice and increased in synaptotagmin4 deficient mice that are protected against diet induced obesity. Research article antiobesity and hypolipidemic activity of.

Mar 28, 2019 it remains to be demonstrated whether longterm caf diet induced obesity induces concurrent physiological and behavioural deficits. Propensity to highfat diet induced obesity in rats is associated with changes in the gut microbiota and gut in. Mekalanos, harvard medical school, boston, ma, and approved november 22, 2006. Together, the data suggest that maternal prenatal stress or highfat diet alters susceptibility of offspring to diet induced obesity and its metabolic consequences. Propensity to highfat dietinduced obesity in rats is. Mar 20, 2019 obesity induced by a highfat diet had a substantial elevation in adiposity parameters, fe, lv, and lvwtibia length when compared to c rats. Mechanisms of the antiobesity effects of oxytocin in dietinduced.

Furthermore, rats cotreated with ginger extract improved the histopathological and ultrastructural renal injury in duced by obesity. A controlled highfat diet induces an obese syndrome in rats. Finally, central ot infusion was shown to cause body weight loss in diet induced obese mice. Diet induced adipocyte number increase in adult rats. Cardiovascular function in a rat model of dietinduced obesity.

For the study of these pathologies, a number of animal models have been developed. Dietinduced obesity alters memory consolidation in female. To extend this line of study we investigated the development of vascular and neural complications in rats fed a high fat diet. Rat models of dietinduced obesity and high fatlow dose. Wistar and sd rats were assigned for 2 experimental groups for 17 weeks. In contrast, the fc was lower in ob than in c rats.

We aimed to investigate the potential efficacy of hmf on preventing obesity and hyperlipidemia in rats fed a highfat diet hfd and its underlying mechanisms. Development of hypertension in a rat model of dietinduced. In our study, one group of rats was fed with standard rat diet, while other group of rats fed with highfat diet for 4 weeks. Gf mice are protected against diet induced obesity. Effect of consumption of a highfat hf diet on body weight, adiposity, and food intake in lowfat lf and hffed animals after 8 or 12 wk on respective diets a. Rats were singly caged with free access to water and subjected to different. Molecules free fulltext soy isoflavones improve the. They were housed in individual metabolic cages nalgene, rochester, ny with a constant temperature and 12 h of light altering with 12 h of darkness. Highfructose and highfat diet induced disorders in rats. The consumption of a highfat diet hfd is considered a risk factor for obesity development. Stern, and jules hirsch rockefeller university, new york, new york 10021 faust, irving m. Modified highsucrose dietinduced abdominally obese and.

When compared over 10 wk of free feeding, hf rats weighed 10% more p. High fatstz rodent models fat fedstz diabetic rodents, developed by combination of diet induced insulin resistance and relatively lowdose streptozotocin stz provide a novel animal model for diet induced type 2 diabetes. Effect of highfat dietinduced obesity on thyroid gland. The dietinduced obesity model dio model is an animal model used to study obesity using animals that have obesity caused by being fed highfat or highdensity diets. We developed a rat model of dietinduced obesity that will be a powerful tool for. Highfatdietinduced obesity causes an inflammatory and tumor. Highfructose and highfat dietinduced disorders in rats. Obese rats model induced by highfat diets were established and gavage treated with 0, 50,150 or 450 mg of sifkg body weightday for 4 weeks. Adrenals were dissected free of fat, weighed to the nearest milligram, and homogenized in a solution of 0. Systemic upregulation of nadph oxidase in dietinduced. However, treating diet induced obese rats with a low dose of.

Pdf highfat dietinduced obesity in animal models researchgate. The physiologic characteristics of the diet induced obesity rat model are insulin resistance, impaired glucose tolerance, dyslipidemia and increased fat deposition. Cqa on lipid metabolism in the liver of spraguedawley rats fed a high. Animals with the most significant weight gain in the dio group were chosen in order to maximize the differences between the two groups. We examined the effects of scfa administration in mice, and show that butyrate, propionate, and acetate all protected against dietinduced obesity and insulin resistance. Ontogeny of dietinduced obesity in selectivelybred rats. Pdf ginger extract ameliorates renal damage in high fat.

Mechanisms of the antiobesity effects of oxytocin in diet. The objective was to determine the effect of diet induced obesity dio on microvascular and neural function. Greater dietinduced obesity in rats consuming sugar solution during the inactive period. Thus, the objective of this study was to evaluate the effect of a chronic 20week caf feeding period on metabolic and cognitive functioning in a spraguedawley rats. Lf, p nov 24, 2016 in contrast, selection from outbred strains presupposes greater variability in both genetic and epigenetic differences, which likely better reflects the susceptibility to obesity in humans. Pdf obesity is rapidly becoming a worldwide epidemic that affects children and adults. Using chowfed lean rats and two groups of highfat diet hfdfed rats with. Highfat dietinduced metabolic syndrome and oxidative stress in. Control c rats had free access to a standard nonpurified diet containing 51% carbohydrate, 4% fat, and 21% protein.

We applied a comparative functional genomics approach to evaluate whether diet. The effect of highfat dietinduced obesity on cardiovascular. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf dietinduced obesity has not been adequately evaluated so far. After 4week induced obesity, the body weight was higher for hed than control rats p obesity and its associated diabetes in humans. Rats fed on the normal diet and hed continued to show increased body weight fig. The major factors that contribute to dietary obesity hyperphagia, energy density and. Induction of obesity by feeding high fat diet experimental obesity was induced by feeding of high fat diet consisting of the following materials corn starch, casein, lard, cholesterol, sodium cholate, soybean oil, coconut oil, wheat bran, mineral mix, vitamin mix were added to 1kg of rat chow diet to rats. Frontiers highfat dietinduced obesity model does not. In group iii, the biochemical parameters and structure of thyroid follicles were improved, and they had a nearnormal appearance. Estradiol replacement improves highfat dietinduced.

Pdf the effect of highfat dietinduced obesity on cardiovascular. Despite the differences between the metabolism and gpr affinities of the three scfas, they ameliorate highfat diet hfdinduced obesity and insulin resistance to a similar extent when given as a dietary supplement 1517. Postnatal diet induced obesity in rats upregulates. Weaning onto highfat diet resulted in obesity and impaired glucose tolerance in offspring exposed to prenatal stress, highfat diet, or both.

Finally, we comment on the reversibility of highfat diet induced obesity. Fat fedstz diabetic rodents, developed by combination of diet induced insulin resistance and relatively lowdose streptozotocin stz provide a novel animal model for diet induced type 2 diabetes. However, the mechanisms underlying such beneficial effects of. Highfat diet hfd induced obesity in rats was an effective model to evaluate anti obesity activities of drugs. Overfeeding induced earlyonset obesity and accelerated the maturation of the hypothalamopituitaryadrenal hpa axis together. When fed ad libitum with a highfat diet hfd, obesity, hyperinsulinemia, hyperglycemia and hypertension establish in these mice. Dietinduced obese dio rat model a unique dio rat model to. From genetics to physiology article pdf available in obesity 202. Obesity is a major health problem worldwide and risk factor for various cardiovascular diseases. Dec 27, 2019 highfat diet induced metabolic syndrome and oxidative stress in obese rats are ameliorated by yogurt supplementation skip to main content thank you for visiting. Group 1 represented the normal control in which the animals were feed on a normal diet npd and had free access to water. These data suggest that the clearance of cholesterol is impaired in op rats, whereas in. Mechanisms underlying the resistance to dietinduced.

Obesity and overweight are associated with atherosclerosis, fatty liver, hyperlipemia, diabetes mellitus, and various types of cancer. The study concluded that the ginger extract used could suppress and decrease the renal damage induced by highfat diet as it possesses potential medicinal values. Increasing dietary leucine intake reduces dietinduced. Dietinduced models for obesity and type 2 diabetes. Energy expenditure in rats with dietinduced obesity article pdf available in american journal of clinical nutrition 442. Antiobesity activity of moringa oleifera leaves against. Excess caloric intake increases the amount of adipose tissue and contributes to metabolic disorders in disrupted metabolic homeostasis. Peripherally infused lean and hfdinduced obese rats received. Table 1 a different types of diet used and dietinduced body and metabolic alterations dietinduced obesity. However, hfd alone induced only transient insulin resistance, glucose intolerance, and hypertriglyceridaemia, without. Effects of ginseng dietary supplementation on a highfat. Nonetheless, a causal role of dietary fat has never been documented, because of inadequate animal models. We sought to determine the net effects of dietary supplementation of leucine on wholebody macronutrient and energy metabolism in mice with free access to a highfat diet hfd and to assess how cardiovascular risks associated with dietinduced obesity may be modified by increased dietary leucine intake.

Antiobesity and hypolipidemic activity of moringa oleifera. However, a direct comparison between wistar and spraguedawley sd rat as models of highfat hf diet induced obesity has not been adequately evaluated so far. Typically mice, rats, dogs, or nonhuman primates are used in these models. Here, our research shows that obesity resulted in spermatogenic degeneration, imbalance of reproductive hormone, testicular oxidative stress and germ cell apoptosis, whereas evidently recovery effects. After rats had ad libitum access to hfd for 6 wk to develop hfd induced obesity and impaired glucose tolerance, we delivered the vector aavshnpy into the dmh of hfd induced obese rats for speci. In the present study our rats also developed a concurrent range of nerve disorders, including sncv slowing, thermal hypoalgesia. In this study, we examined the effects of lactose on longterm highfat diet induced obesity in rats.

Male wistar rats were fed either a high fat diet hfd, 35% fat or a standard diet ct, 3. Cafeteriadiet induced obesity results in impaired cognitive. Custom diet protocols can use any diet manufactured according. Pdf dietinduced obesity in rats leads to a decrease in sperm. As the rats mature, growth rates slow, the gain in body weight and fatfree. The same variables were then evaluated after exposing the rats to a highfat diet, after dio rats selectively developed obesity. Obesity induced by highfat diet in female rats structurally and functionally changed the thyroid gland in a way that may explain hypothyroidism associated with obesity. Rodent model for the study of obesityrelated disorders 387rev assoc med bras 2012. To determine whether gf mice are protected against diet induced obesity, adult c57bl6j males, maintained since weaning on an autoclaved lowfat chow diet 5% lipids. The first group had free access to a standard diet normal diet nd from safe augy, france, with the following macronutrient composition. Modeling dietinduced obesity with obesityprone rats ncbi. The two rat models used in these studies were the diet induced obese and type 2. Mitochondrial oxidative stress in the cardiac alterations.

Therefore, our aim has been to characterize the interactions between cardiolipotoxicity and mitochondrial oxidative stress in the cardiac alterations in diet induced obese rats. Diet induced obesity in sprague dawley rats causes. Highfat dietinduced metabolic syndrome and oxidative stress. Values of a corresponding parameter are significantly different at all time points p diet induced leptin resistance also interferes with memory consolidation in rats, such that marked deficits are observed in object recognition tests in diet induced obese rats compared to wild. These differences were even more marked relative to rats fed the np diet, the control traditionally used in studies of rodent dietinduced obesity. May 28, 20 crosstalk between akkermansia muciniphila and intestinal epithelium controls dietinduced obesity. After 1 week of acclimatization, the rats were randomly divided in two groups. The average food and water intake of each rat are presented in table 1. Pdf dietinduced obesity in spraguedawley rats causes. In this study, we examined the effects of lactose on long. In the aorta, nox4 expression was increased by threefold in obese rats. In our study, one group of rats was fed with standard rat diet, while other group of rats fed with.

Pdf expression of new loci associated with obesity in. Oxidative stress in a rat model of obesityinduced hypertension. A search was made for predisposing factors and sequelae of diet induced obesity dio or resistance to dio dr. A nonobese, dietinduced animal model of nonalcoholic. Effects of dietary lactose on longterm highfatdiet. Measurements were performed of vasodilation in epineurial arterioles by videomicroscopy. Dietinduced adipocyte number increase in adult rats. Combined effect of cromolyn sodium and chlorpheniramine. It was suggested that scfas may regulate gut hormones via their endogenous receptors free fatty acid receptors 2 ffar2 and 3 ffar3, but direct evidence is lacking. Inquire about customized diet induced obese dio models using any of taconics commercial animal models. However, similar plasma triglyceride, plasma free fatty acid and renal. Marques c 1, meireles m2, norberto s2, leite j 2, freitas j 3, pestana d1, faria a4, calhau c.